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anti p stat1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti p stat1
    Anti P Stat1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 517 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+stat1+ser727/Phospho-Stat1+(Ser727)+Antibody/pm41862053-116-55-56
    Average 96 stars, based on 517 article reviews
    anti p stat1 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Regulatory role of POSTN in keloid pathogenesis.
    Article Snippet: Keloids are an inflammatory cutaneous condition, which are characterized by fibroproliferative overgrowth of the skin.. Although keloids are not life‐threatening, their incidence and recurrence are relatively high, thus decreasing the quality of life of patients due to pain, pruritus and cosmetic reasons.. Additionally, the precise molecular mechanisms underlying the pathogenesis of keloids remain largely unexplored, thus limiting the development of therapeutic interventions.

    Article Title: Regulatory role of POSTN in keloid pathogenesis
    Article Snippet: .. The membranes were blocked with blocking buffer (cat. no. ST023; Beyotime Institute of Biotechnology) for 1 h at room temperature, and incubated with primary antibodies (1:1,000) as follows: phosphorylated (p)-STAT1 (Tyr701) (cat. no. 9167), p-STAT1 (Ser727) (cat. no. 8826) and STAT1 (cat. no. 14994; all Cell Signaling Technology, Inc.; β-actin (cat. no. AF5003) was from Beyotime Institute of Biotechnology. ..

    Incubation:

    Article Title: Regulatory role of POSTN in keloid pathogenesis.
    Article Snippet: Keloids are an inflammatory cutaneous condition, which are characterized by fibroproliferative overgrowth of the skin.. Although keloids are not life‐threatening, their incidence and recurrence are relatively high, thus decreasing the quality of life of patients due to pain, pruritus and cosmetic reasons.. Additionally, the precise molecular mechanisms underlying the pathogenesis of keloids remain largely unexplored, thus limiting the development of therapeutic interventions.

    Article Title: Regulatory role of POSTN in keloid pathogenesis
    Article Snippet: .. The membranes were blocked with blocking buffer (cat. no. ST023; Beyotime Institute of Biotechnology) for 1 h at room temperature, and incubated with primary antibodies (1:1,000) as follows: phosphorylated (p)-STAT1 (Tyr701) (cat. no. 9167), p-STAT1 (Ser727) (cat. no. 8826) and STAT1 (cat. no. 14994; all Cell Signaling Technology, Inc.; β-actin (cat. no. AF5003) was from Beyotime Institute of Biotechnology. ..

    other:

    Article Title: Bmal1 haploinsufficiency impairs fear memory and modulates neuroinflammation via the 5-HT2C receptor
    Article Snippet: p-STAT1(Ser727) , Cell Signaling Technology , #8826 , 1:1000.



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    anti p stat1  (Cell Signaling Technology Inc)
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    p stat1 ser727  (Cell Signaling Technology Inc)
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    Knockdown of POSTN blocks the JAK-STAT signaling pathway in KFs. (A) JAK-STAT pathway was revealed to be associated with POSTN knockdown in KFs by gene set enrichment analysis. (B) Heatmap showing DEGs related to the JAK-STAT pathway in si-POSTN and si-CTL KFs. Three independent RNA-sequencing experiments are shown in each group. (C) Reverse transcription-quantitative PCR analysis was performed to validate the DEGs related to the JAK-STAT pathway in si-POSTN- and si-CTL-transfected KFs (n=3). (D) Representative western blotting and semi-quantification of the protein levels of <t>p-STAT1</t> (Tyr701) and p-STAT1 <t>(Ser727)</t> in KFs transfected with si-POSTN and si-CTL in the presence or absence of IFNα +β treatment (n=3). Data are presented as the mean ± SEM. ***P<0.001, **P<0.01, *P<0.05. DEGs, differentially expressed genes; KFs, keloid fibroblasts; NS, not significant; p-, phosphorylated; POSTN, periostin; si, small interfering.
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    Knockdown of POSTN blocks the JAK-STAT signaling pathway in KFs. (A) JAK-STAT pathway was revealed to be associated with POSTN knockdown in KFs by gene set enrichment analysis. (B) Heatmap showing DEGs related to the JAK-STAT pathway in si-POSTN and si-CTL KFs. Three independent RNA-sequencing experiments are shown in each group. (C) Reverse transcription-quantitative PCR analysis was performed to validate the DEGs related to the JAK-STAT pathway in si-POSTN- and si-CTL-transfected KFs (n=3). (D) Representative western blotting and semi-quantification of the protein levels of <t>p-STAT1</t> (Tyr701) and p-STAT1 <t>(Ser727)</t> in KFs transfected with si-POSTN and si-CTL in the presence or absence of IFNα +β treatment (n=3). Data are presented as the mean ± SEM. ***P<0.001, **P<0.01, *P<0.05. DEGs, differentially expressed genes; KFs, keloid fibroblasts; NS, not significant; p-, phosphorylated; POSTN, periostin; si, small interfering.
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    Knockdown of POSTN blocks the JAK-STAT signaling pathway in KFs. (A) JAK-STAT pathway was revealed to be associated with POSTN knockdown in KFs by gene set enrichment analysis. (B) Heatmap showing DEGs related to the JAK-STAT pathway in si-POSTN and si-CTL KFs. Three independent RNA-sequencing experiments are shown in each group. (C) Reverse transcription-quantitative PCR analysis was performed to validate the DEGs related to the JAK-STAT pathway in si-POSTN- and si-CTL-transfected KFs (n=3). (D) Representative western blotting and semi-quantification of the protein levels of p-STAT1 (Tyr701) and p-STAT1 (Ser727) in KFs transfected with si-POSTN and si-CTL in the presence or absence of IFNα +β treatment (n=3). Data are presented as the mean ± SEM. ***P<0.001, **P<0.01, *P<0.05. DEGs, differentially expressed genes; KFs, keloid fibroblasts; NS, not significant; p-, phosphorylated; POSTN, periostin; si, small interfering.

    Journal: Molecular Medicine Reports

    Article Title: Regulatory role of POSTN in keloid pathogenesis

    doi: 10.3892/mmr.2025.13701

    Figure Lengend Snippet: Knockdown of POSTN blocks the JAK-STAT signaling pathway in KFs. (A) JAK-STAT pathway was revealed to be associated with POSTN knockdown in KFs by gene set enrichment analysis. (B) Heatmap showing DEGs related to the JAK-STAT pathway in si-POSTN and si-CTL KFs. Three independent RNA-sequencing experiments are shown in each group. (C) Reverse transcription-quantitative PCR analysis was performed to validate the DEGs related to the JAK-STAT pathway in si-POSTN- and si-CTL-transfected KFs (n=3). (D) Representative western blotting and semi-quantification of the protein levels of p-STAT1 (Tyr701) and p-STAT1 (Ser727) in KFs transfected with si-POSTN and si-CTL in the presence or absence of IFNα +β treatment (n=3). Data are presented as the mean ± SEM. ***P<0.001, **P<0.01, *P<0.05. DEGs, differentially expressed genes; KFs, keloid fibroblasts; NS, not significant; p-, phosphorylated; POSTN, periostin; si, small interfering.

    Article Snippet: The membranes were blocked with blocking buffer (cat. no. ST023; Beyotime Institute of Biotechnology) for 1 h at room temperature, and incubated with primary antibodies (1:1,000) as follows: phosphorylated (p)-STAT1 (Tyr701) (cat. no. 9167), p-STAT1 (Ser727) (cat. no. 8826) and STAT1 (cat. no. 14994; all Cell Signaling Technology, Inc.; β-actin (cat. no. AF5003) was from Beyotime Institute of Biotechnology.

    Techniques: Knockdown, RNA Sequencing, Reverse Transcription, Real-time Polymerase Chain Reaction, Transfection, Western Blot